Dental Stem Cell Biology Research Unit

Dental Stem Cell Biology Research Unit focuses on the cell and molecular biology of stem cells isolated from dental and oral tissues. Mainly, the research aims to elucidate the regulatory pathways controlling stemness maintenance and osteo/odontogenic differentiation. Research Unit is extensively working on Notch, Wnt, TGF, and FGF signaling in various stem cells, including dental pulp stem cells, periodontal ligament stem cells, stem cells isolated from human exfoliated deciduous teeth, stem cells isolated from apical papilla, and induced pluripotent stem cells.

Focus Area
  • Notch signaling in dental stem cells
  • Biology of dental pulp and regenerative endodontics
  • Regulation of osteogenic differentiation in induced pluripotent stem cells
  • Decellularized extracellular matrix and its role in dental tissue regeneration

Principle Investigator 

Prof. Dr. Thanaphum Osathanon
Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, 34 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand
Tel.: (+66) 2218-8885
E-mail: thanaphum.o@chula.ac.th
Selected Publications (2016-2020)
  1. Manokawinchoke J, Pavasant P, Limjeerajarus CN, Limjeerajarus N, Osathanon T, Egusa H. Mechanical loading and the control of stem cell behavior. Arch Oral Biol. 2021 Feb 24;125:105092. doi: 10.1016/j.archoralbio.2021.105092.
  2. Nantavisai S, Pisitkun T, Osathanon T, Pavasant P, Kalpravidh C, Dhitavat S, Makjaroen J, Sawangmake C. Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp. Sci Rep. 2020 Nov 26;10(1):20703.
  3. Kuncorojakti S, Rodprasert W, Yodmuang S, Osathanon T, Pavasant P, Srisuwatanasagul S, Sawangmake C. Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells. J Biol Eng. 2020 Aug 24;14:23.
  4. Manokawinchoke J, Sumrejkanchanakij P, Boonprakong L, Pavasant P, Egusa H, Osathanon T. NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells. Sci Rep. 2020 Aug 7;10(1):13329.
  5. Sawangmake C, Rodprasert W, Osathanon T, Pavasant P. Integrative protocols for an in vitro generation of pancreatic progenitors from human dental pulp stem cells. Biochem Biophys Res Commun. 2020 Sep 10;530(1):222-229.
  6. Nowwarote N, Manokawinchoke J, Kanjana K, Fournier BPJ, Sukarawan W, Osathanon T. Transcriptome analysis of basic fibroblast growth factor treated stem cells isolated from human exfoliated deciduous teeth. 2020 Jun 25;6(6):e04246.
  7. Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, Egusa H. Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs. Stem Cells Int. 2020 May 14;2020:7082679.
  8. Limjeerajarus N, Fakkao M, Lampang SN, Osathanon T, Pavasant P, Limjeerajarus CN. Experimental data on mechanical behavior and numerical data on tensile stress distribution of a hyperelastic Polydimethysiloxane (PDMS) based membrane for cell culture. Data Brief. 2020 Apr 7;30:105476.
  9. Kornsuthisopon C, Pirarat N, Osathanon T, Kalpravidh C. Autologous platelet-rich fibrin stimulates canine periodontal regeneration. Sci Rep 2020l10(1):1850.
  10. Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, Egusa H. In vitro fabrication of hybrid bone/cartilage complex using mouse induced pluripotent stem cells. Int J Mol Sci 2020;21(2):piiE581.
  11. Manaspon C, Boonprakong L, Porntaveetus T, Osathanon T. Preparation and characterization of Jagged1-bound fibrinogen-based microspheres and their cytotoxicity against human dental pulp cells. J Biomater Appl J Biomater Appl. 2020 Mar;34(8):1105-1113.
  12. Hansamuit K, Osathanon T, Suwanwela J. Effect of Jagged1 on the expression of genes in regulation of osteoblast differentiation and bone mineralization ontology in human dental pulp and periodontal ligament cells. J Oral Bio and Cranio res 2020 [Epub ahead of print]
  13. Nantavisai S, Egusa H, Osathanon T, Sawangmake C. Mesenchymal stem cell-based bone tissue engineering for veterinary practice. Heliyon 2019;5(11):e02808.
  14. Nowwarote N, Osathanon T, Kanjana K, Theerapanon T, Porntaveetys T, Shotelersuk V. Decrease osteogenic activity and mineralization of alveolar bone cells from a patient with amelogenesis imperfecta and FAM83H 1261G>T mutation. Genes Dis 2019;6(4):391-397.
  15. Manokawinchoke J, Pavasant P, Sawangmake C, Limjeerajarus N, Limjeerajarus CN, Egusa H, Osathanon T. Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor beta pathway. Cell Death Dis 2019;10(10):761.
  16. Manokawinchoke J, Pavasant P, Sawangmake C, Limjeerajarus N, Limjeerajarus CN, Egusa H, Osathanon T. RNA sequencing data of human periodontal ligament cells treated with continuous and intermittent compressive force. Data Brief 2019;26:104553.
  17. Yaemkleebbua K, Osathanon T, Nowwarote N, Limjeerajarus CN, Sukarawan W. Analysis of hard tissue regeneration and Wnt signaling in dental pulp tissues after direct pulp capping with different materials. Int Endod J. 2019 doi:10.1111/iej.13162 [Epub ahead of print]
  18. Nowwarote N, Osathanon T, Kanjana K, Theerapanon T, Porntaveetus T, Shotelersuk V. Decreased osteogenic activity and mineralization of alveolar bone cells from a patient with amelogenesis imperfecta and FAM83H 1261G>T mutation. Genes and Diseases 2019 doi:10.1016/j.gendis.2019.07.005 [Epub ahead of print]
  19. Manaspon C, Thaweesapphithak S, Osathanon T, Suphapeetiporn K, Porntaveetus T, Shotelersuk V. A novel de novo mutation substantiates KDF1 as a gene causing ectodermal dysplasia. Br J Dermatol 2019;181:419-420.
  20. Suwanwela J, Hansamuit K, Manokawinchoke J, Sa-Ard-Iam N, Mahanonda R, Pavasant P, Osathanon T. Gene expression profiling of Jagged1-treated human periodontal ligament cells. Oral Dis 2019;25:1203-1213.
  21. Osathanon T, Manokawinchoke J, Sa-Ard-Iam N, Mahanonda R, Pavasant P, Suwanwela J. Jagged1 promotes mineralization in human bone-derived cells. Arch Oral Biol 2019;99:134-140.
  22. Charoenpong H, Osathanon T, Pavasant P, Limjeerajarus N, Keawprachum B, Limjeerajarus CN, Cheewinthamrongrod V, Palaga T, Lertchirakarn V, Ritprajak P. Mechanical stress induced S100A7 expression in human dental pulp cells to augment osteoclast differentiation. Oral Dis. 2019 Apr;25(3):812-821.
  23. Nowwarote N, Sukarawan W, Pavasant P, Foster BL,Osathanon T. Basic fibroblast growth factor regulates phosphate/pyrophosphate regulatory genes in stem cells isolated from human exfoliated deciduous teeth. Stem Cell Res Ther. 2018 Dec 10;9(1):345.
  24. Nowwarote N, Sukarawan W, Kanjana K, Pavasant P, Fournier BPJ,Osathanon T. Interleukin 6 promotes an in vitro mineral deposition by stem cells isolated from human exfoliated deciduous teeth. R Soc Open Sci. 2018 Oct 31;5(10):180864.
  25. Nowwarote N, Theerapanon T,Osathanon T, Pavasant P, Porntaveetus T, Shotelersuk V. Amelogenesis imperfecta: A novel FAM83H mutation and characteristics of periodontal ligament cells. Oral Dis. 2018 Nov;24(8):1522-1531.
  26. Manokawinchoke J, Nattasit P, Thongngam T, Pavasant P, Tompkins KA, Egusa H,Osathanon T. RNA sequencing data of Notch ligand treated human dental pulp cells. Data Brief. 2018 Jan 31;17:407-413.
  27. Porntaveetus T, Nowwarote N,Osathanon T, Theerapanon T, Pavasant P, Boonprakong L, Sanon K, Srisawasdi S, Suphapeetiporn K, Shotelersuk V. Compromised alveolar bone cells in a patient with dentinogenesis imperfecta caused by DSPP mutation. Clin Oral Investig. 2019 Jan;23(1):303-313.
  28. Nowwarote N, Chanjavanakul P, Kongdecha P, Clayhan P, Chumprasert S, Manokawinchoke J, Egusa H, Pavasant P,Osathanon T. Characterization of a bioactive Jagged1-coated polycaprolactone-based membrane for guided tissue regeneration. Arch Oral Biol. 2018 Apr;88:24-33.
  29. Suwanwela J,Osathanon T. Inflammation related genes are upregulated in surgical margins of advanced stage oral squamous cell carcinoma. J Oral Biol Craniofac Res. 2017 Sep-Dec;7(3):193-197.
  30. Porntaveetus T,Osathanon T, Nowwarote N, Pavasant P, Srichomthong C, Suphapeetiporn K, Shotelersuk V. Dental properties, ultrastructure, and pulp cells associated with a novel DSPP mutation. Oral Dis. 2018 May;24(4):619-627.
  31. Manokawinchoke J, Nattasit P, Thongngam T, Pavasant P, Tompkins KA, Egusa H,Osathanon T. Indirect immobilized Jagged1 suppresses cell cycle progression and induces odonto/osteogenic differentiation in human dental pulp cells. Sci Rep. 2017 Aug 31;7(1):10124. 
  32. Manokawinchoke J, Pavasant P,Osathanon T. Intermittent compressive stress regulates Notch target gene expression via transforming growth factor-β signaling in murine pre-osteoblast cell line. Arch Oral Biol. 2017 Oct;82:47-54.
  33. Manokawinchoke J, Sumrejkanchanakij P, Pavasant P, Osathanon T. Notch signaling participates in TGF-beta-induced SOST expression under intermittent compressive stress. J Cell Physiol. 2017 Aug;232(8):2221-2230.
  34. Osathanon T, Manokawinchoke J, Egusa H, Pavasant P. Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells. J Oral Sci. 2017;59(3):405-413.
  35. Ketkaew Y, Osathanon T, Pavasant P, Sooampon S. Apigenin inhibited hypoxia induced stem cell marker expression in a head and neck squamous cell carcinoma cell line. Arch Oral Biol. 2017;74:69-74.
  36. Nowwarote N, Sukarawan W, Pavasant P, Osathanon T. Basic fibroblast growth factor regulates REX1 expression via IL-6 in stem cells isolated from human exfoliated deciduous teeth. J Cell Biochem 2017;118(6):1480-1488.
  37. Rattanawarawipa P, Pavasant P, Osathanon T, Sukarawan W. Effects of lithium chloride on cell proliferation and osteogenic differentiation in stem cells from human exfoliated deciduous teeth. Tissue Cell 2016;48:425-431.
  38. Manokawinchoke J, Osathanon T, Pavasant P. Regulation of osteoprotegerin expression by Notch singling in human oral squamous cell carcinoma cell line. Asian Pac J Tropical Biomed. 2016;6:692-697.
  39. Manokawinchoke J, Osathanon T, Egusa H, Pavasant P. Hypoxia enhances osteogenic differentiation in retinoid acid-treated murine induced pluripotent stem cells. Tissue Eng Regen Med 2016 13(5): 547–553.
  40. Osathanon T, Nowwarote N, Pavasant P. Expression and influence of Notch singling in oral squamous cell carcinoma. J Oral Sci. 2016;58:283-94.
  41. Osathanon T, Sawangmake C, Ruangchainicom N, Wutikornwipak P, Kantukiti P, Nowwarote N, Pavasant P. Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface. J Oral Biol Craniofac Res. 2016;6:2-9.
  42. Sukarawan W, Peetiakarawach K, Pavasant P, Osathanon T. Effect of Jagged-1 and Dll-1 on osteogenic differentiation by stem cells from human exfoliated deciduous teeth. Arch Oral Biol. 2016;65:1-8.
  43. Manokawinchoke J, Sumrejkanchanakij P, Subbalekha K, Pavasant P, Osathanon T. Jagged1 inhibits osteoprotegerin expression by human periodontal ligament cells. J Periodontal Res. 2016 Dec;51(6):789-799.
  44. Wang F, Okawa H, Kamano Y, Niibe K, Kayashima H, Osathanon T, Pavasant P, Saeki M, Yatani H, Egusa H. Controlled Osteogenic Differentiation of Mouse Mesenchymal Stem Cells by Tetracycline-Controlled Transcriptional Activation of Amelogenin. PLoS One. 2015 Dec 28;10(12):e0145677.
  45. Chuenjitkuntaworn B, Osathanon T, Nowwarote N, Supaphol P, Pavasant P. The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering. J Biomed Mater Res A. 2016 Jan;104(1):264-71.
  46. Osathanon T, Nowwarote N, Pavasant P, Sukarawan W. Influence of Jagged1 on apoptosis-related gene expression: a microarray database analysis. Genes Genom. 2015;10:837-843.
  47. Nowwarote N, Sawangmake C, Pavasant P, Osathanon T. Review of the role of basic fibroblast growth factor in dental tissue-derived mesenchymal stem cells. Asian Biomed 2015;9:271-283.
  48. Nowwarote N, Pavasant P, Osathanon T. Role of endogenous basic fibroblast growth factor in stem cells isolated from human exfoliated deciduous teeth. Arch Oral Biol. 2015 Mar;60(3):408-15.
  49. Govitvattana N, Osathanon T, Toemthong T, Pavasant P. IL-6 regulates stress-induced REX-1 expression via ATP-P2Y1 signalling in stem cells isolated from human exfoliated deciduous teeth. Arch Oral Biol. 2015 Jan;60(1):160-6.
  50. Osathanon T, Vivatbutsiri P, Sukarawan W, Sriarj W, Pavasant P, Sooampon S. Cobalt chloride supplementation induces stem-cell marker expression and inhibits osteoblastic differentiation in human periodontal ligament cells. Arch Oral Biol. 2015 Jan;60(1):29-36.
  51. Manokawinchoke J, Ritprajak P, Osathanon T, Pavasant P. Estradiol induces osteoprotegerin expression by human dental pulp cells. Odontology. 2016;104(1):10-8.